Zein Barakat
This study investigates a one-step reaction strategy for quantifying creatinine via an enzymatic activity using a personal glucose meter (PGM). Creatinine is a waste product that is filtered through the kidneys. To be able to detect creatinine levels at an early stage, allows for an early diagnosis of kidney malfunction. Easy and noninvasive detection of creatinine in urine is diagnostically favorable. A PGM is commercially available; however, it can only detect a single target, glucose. A link between the functionality of a PGM, an enzymatic reaction, and a specific antibody-target interaction could enable the quantification of non-glucose targets. Invertase is one enzyme that catalyzes the breakdown of sucrose into glucose and fructose, and it can form a link between the detection of glucose and non-glucose targets.