Alexander Petrovich Lykov, Olga Vladimirovna Poveshchenko, Natalia Anatollievna Bondarenko, Maria Alexandrovna Surovtseva
Fibroblasts and platelet-rich plasma (PRP) used in regenerative medicine. This study evaluated whether fibroblast alone or in combination with PRP promote burn skin wound healing in vivo. Twenty CBA male mice were divided to 4 treatment groups: control (0.05 mL of phosphate-buffered saline, intradermal), PRP (0.05 mL of PRP, intradermal), fibroblasts/PRP (4x104 fibroblast in 0.05 mL PRP, intradermal), and fibroblasts (4x104 fibroblast in 0.05 mL PBS, intradermal), immediately after burning with a metal rod (100 °C for 40 s). The healing process was evaluated by gross examination throughout the experimental period on day 4, 11, 14 and 21, and histologic examination on day 21. Cytokines, growth factors and NO were determined in PRP by ELISA. The results indicated that the combination fibroblasts with PRP significantly promote wound healing (p ≤ 0.05). Histological study on day 21 revealed that fibroblast/PRP-treated wounds showed enhanced reepithelialization, sebaceous gland, hair follicles, and blood vessels formation. PRP contains wide-range of cytokines, growth factors, and NO. The current results demonstrated that fibroblasts in combination with PRP possesses beneficial burn skin wound healing.